Circular dichroism and DNA secondary structure
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چکیده
منابع مشابه
Circular dichroism and DNA secondary structure.
The change in average rotation of the DNA helix has been determined for the transfer from 0.05 M NaCl to 3.0 M CsCl, 6.2 M LiCl and 5.4 M NH4Cl. This work, combined with data at lower salt from other laboratories, allows us to relate the intensity of the CD of DNA at 275 nm directly to the change in the number of base pairs per turn. The change in secondary structure for the transfer of DNA fro...
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Tatiana C. Bicudo, Rogério C. Bicudo, Lucimara A. Forato, Leila M. Beltramini, Luiz A. R. Batista, Rubens Bernardes Filho, Luiz A. Colnago 1 Universidade de São Paulo, Instituto de Quı́mica de São Carlos, Av. Trabalhador Sãocarlense, 400, São Carlos, SP 13560-970, Brazil 2 Universidade Federal da Paraı́ba, Departamento de Quı́mica, Cidade Universitária, Campus I, CEP 58059-900, João Pessoa, Brazil...
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A curated library of circular dichroism spectra of 23 G-quadruplexes of known structure was built and analyzed. The goal of this study was to use this reference library to develop an algorithm to derive quantitative estimates of the secondary structure content of quadruplexes from their experimental CD spectra. Principal component analysis and singular value decomposition were used to character...
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Here we review studies that provided important information about conformational properties of DNA using circular dichroic (CD) spectroscopy. The conformational properties include the B-family of structures, A-form, Z-form, guanine quadruplexes, cytosine quadruplexes, triplexes and other less characterized structures. CD spectroscopy is extremely sensitive and relatively inexpensive. This fast a...
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Recent developments in instrumentation and bioinformatics show that the technique of synchrotron radiation circular dichroism spectroscopy can provide novel information on protein secondary structures and folding motifs, and has the potential to play an important role in structural genomics studies, both as a means of target selection and as a high-throughput, low-sample-requiring screening met...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1979
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/6.2.797